Effects of Ultradry Treatment on the Ultrastructure of Seed Biofilms and Organelles

Effects of Ultradry Treatment on Seed Biofilms Using the seed benches at the Top Research Institute to study the seed samples, it was found that the metabolic activity of the cells was reduced as the seed vigor was reduced. Among the factors that regulate cell metabolism, the metabolic components The spatial separation and the correct combination of metabolic complexes are very important, and the membrane has an extremely important role in intracellular localization. Once the membrane system is damaged, the organelles will be distorted and disintegrated, and various macromolecular enzymes will be destroyed. Causes metabolic abnormalities and accelerates loss of seed viability and vitality.
The excessive intracellular electrolyte leakage during the decline of seed vigor is an important indicator of biofilm damage. Super soaked seeds such as rapeseed, peanut, eucalyptus, sorghum, onion, etc. can directly cause soaking of large amounts of substances in the cells. The extravasation of electrolytes in the cells after strict backwater treatment is reduced, and there is no significant difference compared with the control. It indicates that the backwater has a good effect on the physical repair of the ultra-dry seed film, and the ultra-dry itself does not cause damage to the film. After storage for a certain period of time, the extravasation amount of ultra-dry seeds intracellular electrolyte was significantly lower than that of non-ultra-dried seeds, indicating that the ultra-dry seed membrane was better preserved than non-ultra-dried seeds during storage. After ultra-dry treatment of Chinese cabbage and eucalyptus seeds, the membrane fluidity was maintained to some extent under artificial or natural storage conditions. This may be because the ultra-dry storage maintains the random rotation of the hydrocarbon chain molecules in the phospholipid bilayer on the membrane, so that the inherent properties of the molecular arrangement are maintained and the membrane lipid is prevented from forming a gel phase, so that the membrane fluidity can be maintained and the membrane flow can be maintained. Sex is a necessary condition for cells to carry out life activities.
H+-ATPase is a specific enzyme located on the plasma membrane and plays an important role in energy conversion during seed germination. Its activity level is closely related to seed vigor. High-vibration seeds have higher H`-ATP Enzymatic activity. The content and activity of H 10 ATPase in ultra-dried seeds during storage can be maintained well, and the content and activity of non-ultra-dried seed H`-ATPase are affected. The mitochondria also have the same Na-K ATPase. the result of. This change in membrane protein in turn suggests that the physical state of the ultra-dried seed membrane during storage is more complete than that of the non-ultra dry seed membrane.
The effects of ultra-dry treatment on the ultrastructure of seed organelles The reasons for seed ageing and deterioration are complex. It is generally believed that when the seeds are deteriorated, the cytological deterioration phenomenon also occurs. The first manifestation is the ultrastructural changes of the apical meristem. Embryos that are generally mildly aged but still have germination power often exhibit local vesicular uplift of the nucleus, deformation of mitochondria and plastids, deformation and reduction of Golgi apparatus, and slowing down of ribosome synthesis. In the case of severe deterioration, the membrane structure is swollen and disordered, and the spheroid is broken. In the cell, it fuses into clusters and arranges along the wall. The starch grains in the plastids divide, and some disappear.
Transmission electron microscopy was used to observe the root tip cells of the non-ultra-dried seedlings on the old onion. It was found that the nuclear membrane boundary was not clear, some nuclei had pinocytosis, some nuclei were disintegrated, mitochondria were deformed, liposomes were fused and arranged. Irregular, electron dense material (residue after fat consumption) appears. However, the ultra-dry seed organelles that were equally aged had normal development and were distinguished from the ultrastructure characteristics of the root tip cells of the deficient seed embryos. Similar results were also seen in ultradry seed storage of canola, peanuts, soybeans, and wheat. Observation of the external structure of seed coat cells by scanning electron microscopy revealed that the arrangement of epidermal cells of the super-dried seeds changed, but returned to normal after returning to water. Although some seeds such as soybeans have a vegetative phenomenon, they are hygroscopic and dry before ultra-dry. After treatment with polyethylene glycol (PEG), this phenomenon can be avoided. From the inside to the outside, the ultra-dry does not have an adverse effect on the preservation of the seed structural integrity, and the embryo cells have an increased anti-aging ability.

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