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The length of the column: because the resolution is proportional to the square root of the column length. Longer columns increase resolution. In general: 15m short columns are used for rapid separation of simpler samples, but also for rapid component analysis; 30m columns are the most commonly used column lengths; most analyses are performed on columns of this length; 50m, 60m Or longer columns are used to separate more complex samples.
For the choice of caliber: The diameter of the column directly affects the column's efficiency, retention characteristics, and sample size. Small-caliber columns have higher efficiency than large-caliber columns, but the column capacity is smaller.
0.25mm: High column efficiency, low column capacity. It is better to separate complex samples.
0.32mm: The column efficiency is slightly lower than the 0.25mm column, but the column capacity is about 60% higher.
0.53mm: With column capacity similar to packed column, it can be used for split injection or splitless injection. When column capacity is the main consideration (such as trace analysis), it is more suitable to choose large-caliber capillary column. Experts believe that for the average user, a capillary of 30 meters or 50 meters in length can be selected, and the diameter of 0.32 mm is more convenient.
Gas column configuration experience sharing
The experience of GC column configuration is very common. The friend who uses the gas chromatograph for the first time does not know how to configure the column properly. He always hopes that the more columns, the better. He blindly purchases many columns or fixatives, supports, etc., resulting in many idle results. waste. The experts hereby recommend some worthwhile experiences for everyone.