Why test the data of photosynthesis under the condition of empty leaf chamber

The photosynthesis instrument can be measured in the leaf chamber (reactor) or directly used outdoors, so it can be measured in vivo and in vitro. The photosynthesis instrument is matched with various types of leaf chambers (reactors), and can be applied to various measurement objects such as plant leaves, seeds, insects, etc. It can be said that the leaf chamber plays an important auxiliary role in the determination and analysis of photosynthesis. In many measurement experiments, technicians will test the data of the photosynthesis under the conditions of the empty leaf chamber. Why?

"Empty leaf chamber" means that no leaves are added to the leaf chamber. This measurement is mainly to test the net photosynthetic rate A of the photosynthesis instrument under the conditions of "empty leaf chamber". The net photosynthetic rate value A of the "empty leaf chamber" can reflect the performance of the entire photosynthesis measurement system. Why do you say that? This starts with how the net photosynthetic rate A is derived.

Usually, we use the "differential" measurement method to calculate the net photosynthetic rate A of the leaves. As shown in the following formula, A is calculated by measuring the CO2 concentration in the reference chamber and the sample chamber and using the difference. In the measurement, it is necessary to ensure the stability of the system air intake, which is reflected by CO2R; it is also necessary to ensure that the vane cavity does not leak air (LI-6800 adopts appropriate boosting technology), which is reflected by CO2S; due to these two values Two sets of analyzers are used to measure separately, and the consistency of the two must be ensured. For example, under the condition of an empty leaf chamber, the theoretical value of the difference between the two is zero.

Differential

Using this, we can effortlessly measure the photosynthetic gas exchange of "featherweight" samples, such as shaded plant leaves, tiny leaves, or dark respiration rate.

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