A new method for the detection of residual fipronil in eggs developed by the Technical Biotechnology Institute

[ Instrument network instrument development ] Fipronil (fipronil) is a broad-spectrum, high-efficiency and long-lasting phenylpyrazole insecticide, which has been widely used in seed treatment, pest control and animal health protection. However, the use of fipronil insecticides can lead to egg contamination, which is toxic to humans. At present, the use of gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and other traditional methods to detect fipronil in eggs is complicated, but the sensitivity is not sufficient by ordinary Raman spectroscopy.
Recently, Professor Huang Qing of the Institute of Technical Biology has developed a new label-free surface-enhanced Raman spectroscopy (SERS) method, which can rapidly detect fipronil residues in eggs based on SiO2@Au core/shell nanoparticles. The relevant results were published in the analytical chemistry journal Talanta.
The research group prepared a highly sensitive translucent SERS substrate with uniform assembly of SiO2@Au nanoparticles. The fipronil-contaminated egg membrane was directly placed on the substrate, and the SERS measurement was directly performed. The SERS spectrum of fipronil residue was obtained. Bands such as 1583, 1422, 1307 and 867 cm-1 appeared in the model; the structure and spectrum of fipronil molecules were calculated by density functional theory (DFT), and the Raman spectra of these features were identified and verified.
Studies have shown that measurements and analysis at low levels of 10-7M can be achieved. This work also provides new ideas and solutions for detecting pesticides or other contaminants in foods other than eggs.

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